VOLUME 5 NUMBER 1 (January to June 2012)


Philipp. Sci. Lett. 2012 5 (1) 046-052
available online: March 09, 2012

*Corresponding author
Email Address: consolacion.ragasa@dlsu.edu.ph
Submitted: September 22, 2011
Revised: October 21, 2011
Accepted: December 27, 2011


Cytotoxic Isothiocyanates from Moringa oleifera Lam Seeds

by Consolacion Y. Ragasa1,*, Ruel M. Levida1, Ming-Jaw Don2 and Chien-Chang Shen2

1 Chemistry Department and Center for Natural Sciences and Ecological Research,
   De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines
2 National Research Institute of Chinese Medicine, 155-1, Li-Nong St., Sec. 2,
   Taipei 112, Taiwan
The dichloromethane extract of the freeze-driedseeds of Moringa oleifera Lam afforded 4-(4'-O-acetyl-α-L-rhamnosyloxy) benzyl isothiocyanate(1), 4-(α-L-rhamnosyloxy) benzyl isothiocyanate(2), squalene (3) and sitosterol (4). Thedichloromethane extract of the air-dried leaves of M. oleiferaafforded polyprenol (5), phytyl fatty acid ester (6) and lutein (7).The structures of 1 and 2 were elucidated by extensive 1D and2D NMR spectroscopy, while those of 3-7 were identified bycomparison of their 13C NMR data with those reported in theliterature.

Isothiocyanates 1 and 2 were evaluated for cytotoxicityagainst the human cancer cell lines, non-small cell lungadenocarcinoma (A549) and colon carcinoma (HCT 116), andthe non-cancer cell line Chinese hamster ovary cells (AA8)using the MTT cytotoxicity assay. Compounds 1 and 2 werecytotoxic against A549 with IC50 values of 10 and 12 µM,respectively, while the positive control, Doxorubicin gave anIC50 value of 4 µM. Isothiocyanates 1 and 2 were also cytotoxicagainst HCT 116 with IC50 values of 12 and 11 µM, respectivelywhile Doxorubicin exhibited an IC50 value of 3 µM. Compounds1, 2 and Doxorubicin were also cytotoxic against AA8 with IC50values of 22, 15 and 4 µM, respectively.

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