Unveiling the genomic landscape: Whole genome draft and phenotypic profiling of <i>Oceanobacillus</i> sp. SE10311 isolated from Mount Makiling, Philippines

Noel H. Tan Gana; Cernan P. Ruz; Eufrocinio C. Marfori; Edwin P. Alcantara; Maria Genaleen Q. Diaz; Lucille C. Villegas; Eldrin DLR. Arguelles; Armi R. Creencia; Neil H. Tan Gana; Rosario G. Monsalud; Rina B. Opulencia

VOLUME 17 (Supplement)

SciEnggJ%202024%20Special%20Issue%20148 154 Maarof%20et%20al

SciEnggJ 17 (Supplement) 304-320
available online: July 26, 2024
DOI: https://doi.org/10.54645/202417SupNVF-47

*Corresponding author
Email Address: nhtangana@up.edu.ph
Date received: January 5, 2024
Date revised: April 16, 2024
Date accepted: June 30, 2024

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ARTICLE

Unveiling the genomic landscape: Whole genome draft and phenotypic profiling of Oceanobacillus sp. SE10311 isolated from Mount Makiling, Philippines

Noel H. Tan Gana*¹, Cernan P. Ruz1 ¹, Eufrocinio C. Marfori¹, Edwin P. Alcantara¹, Maria Genaleen Q. Diaz², Lucille C. Villegas², Eldrin DLR. Arguelles¹, Armi R. Creencia¹, Neil H. Tan Gana^3,4, Rosario G. Monsalud¹, and Rina B. Opulencia²

¹National Institute of Molecular Biology and Biotechnology,
     University of the Philippines Los Baños
²Institute of Biological Sciences, College of Arts and Sciences,
     University of the Philippines Los Baños
³Department of Biochemistry and Molecular Biology, College of
     Medicine, University of the Philippines Manila
⁴Department of Arts and Sciences, City College of Calamba

KEYWORDS: Oceanobacillus, antimicrobial activity, Illumina sequencing technology, cellulase activity

The genus Oceanobacillus remains one of the least understood groups of Bacillaceae due to limited information and a scarcity of accessions. With the full genome sequencing and partial phenotypic characterization of the cellulase and brown-pigment-producing Oceanobacillus sp. SE10311, an isolate from Mount Makiling, this study provided taxonomic, genomic, and functional insights into this bacterial discovery. The results showed that Oceanobacillus sp. SE10311 draft genome contained 3.946 million base pairs assembled from 137 contigs and 39.30 percent G+C. The whole genome phylogeny and pangenome analyses revealed the identity of Oceanobacillus sp. SE10311 as an isolate of Oceanobacillus indicireducens with greater than 98% genome similarity to the type strain of the species. The confirmed presence of the associated genes from the genome annotation rationalized the pigmentation and cellulose degradation of Oceanobacillus sp. SE10311. This research milestone presents Oceanobacillus sp. SE10311, with distinct chromogenic and cellulolytic properties as a completed whole genome-sequenced bacterial accession from the Philippines.