Isolation of griseofulvin from the endolichenic fungus <i>Cubamyces menziesii</i> (Berk.) Lücking inhabiting <i>Parmotrema rampoddense</i> (Nyl.) Hale

Mario A. Tan; Jake Darryn dela Cruz; Hilbert D. Magpantay; Carlo Chris S. Apurillo; Thomas Edison E. dela Cruz

VOLUME 17 (Supplement)

SciEnggJ%202024%20Special%20Issue%20148 154 Maarof%20et%20al

SciEnggJ 17 (Supplement) 432-442
available online: November 18, 2024
DOI: https://doi.org/10.54645/202417SupKXN-49

*Corresponding author
Email Address: matan@ust.edu.ph; tedelacruz@ust.edu.ph
Date received: August 11, 2024
Date revised: September 4, 2024
Date accepted: September 6, 2024

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ARTICLE

Isolation of griseofulvin from the endolichenic fungus Cubamyces menziesii (Berk.) Lücking inhabiting Parmotrema rampoddense (Nyl.) Hale

Mario A. Tan*^1,2, Jake Darryn dela Cruz¹, Hilbert D. Magpantay³, Carlo Chris S. Apurillo^4,5, and Thomas Edison E. dela Cruz*^1,2

¹College of Science, University of Santo Tomas, España Blvd.
     1015 Manila, Philippines
²Research Center for the Natural and Applied Sciences,
     University of Santo Tomas, España Blvd. 1015 Manila, Philippines
³Chemistry Department, De La Salle University, 2401 Taft Avenue,
     0933 Manila, Philippines
⁴Department of Science and Technology - Center for Research in
     Science and Technology (CReST), Philippine Science High
     School - Eastern Visayas Campus, 6500 Leyte, Philippines
⁵Center of Excellence in Fungal Research, Mae Fah Luang
     University, 57100 Chiang Rai, Thailand

KEYWORDS: Cubamyces, Endolichenic fungi, Griseofulvin, Trametes

Efungi represent valuable sources for the discovery of diverse and biologically relevant natural products with significant applications in pharmaceuticals, agriculture, and industry. In this study, Cubamyces menziesii (Berk.) Lücking, an endolichenic fungus isolated from the lichen Parmotrema rampoddense (Nyl.) Hale, was subjected to extraction and chromatographic purification to obtain griseofulvin (1) and acetyl tributyl citrate (2). The antimicrobial activity of the isolated compounds was assessed using the microtiter plate antimicrobial assay showing 125 – 250 µg/mL minimum inhibitory concentration and minimum bactericidal concentration in Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 25923. This is also the first report of griseofulvin (1), a commercially available antifungal drug, from the genus Cubamyces. The findings highlight the potential of C. menziesii as a promising resource for bioactive compounds, with implications for the development of novel antimicrobial agents for medical and industrial applications.