Response surface methodology-based optimization of cellulase production by <i>Trichoderma asperelloides</i> RS1 through solid-state fermentation using coconut coir

Jhoanna Mae A. Minion; Leo Joseph F. Labitag; Annie Cita T. Kagaoan; Adonis A. Yanos; Sheryl Lozel B. Arreola; Nico G. Dumandan

VOLUME 18 NUMBER 1 (January to June 2025)

PSL%202021 vol14-no01-p12-28-Mikita%20and%20Padlan

SciEnggJ. 2025 18 (1) 051-063
available online: March 31, 2025
DOI: https://doi.org/10.54645/2025181GGL-47

*Corresponding author
Email Address: ngdumandan@up.edu.ph
Date received: October 22, 2024
Date revised: November 28, 2024
Date accepted: January 3, 2025

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ARTICLE

Response surface methodology-based optimization of cellulase production by Trichoderma asperelloides RS1 through solid-state fermentation using coconut coir

Jhoanna Mae A. Minion¹, Leo Joseph F. Labitag², Annie Cita T. Kagaoan², Adonis A. Yanos¹, Sheryl Lozel B. Arreola¹, and Nico G. Dumandan*²

¹Institute of Chemistry, College of Arts and Sciences, University of
the Philippines Los Baños, College 4031, Laguna
²National Institute of Molecular Biology and Biotechnology,
University of the Philippines Los Baños, College 4031, Laguna

KEYWORDS: Trichoderma asperelloides, cellulase, coconut coir, response surface methodology, solid state fermentation

The study optimized cellulase production from Trichoderma asperelloides RS1 using coconut coir as a substrate through solid-state fermentation. A Box-Behnken response surface methodology was employed to optimize three independent factors: fermentation time (3, 6.5, and 10 days), initial media pH (4, 6, and 8), and initial moisture content (80%, 140%, and 200%). Response surface methodology showed significant models for FPase and CMCase activities with p-values <0.0001. Optimal FPase activity (0.762 U_glc/gds) was achieved at pH 6.0, 140% moisture, and 6.5 days. The highest CMCase activity (0.295 U_glc/gds) occurred after 3 days at pH 8.0 and 140% moisture. The regression model showed strong agreement between predicted and actual cellulase activities, with percent errors of 0.74% and 1.83% for FPase and CMCase, respectively.