VOLUME 18 NUMBER 1 (January to June 2025)

PSL%202021 vol14-no01-p12-28-Mikita%20and%20Padlan

SciEnggJ. 2025 18 (1) 051-063
available online: March 31, 2025
DOI: https://doi.org/10.54645/2025181GGL-47

*Corresponding author
Email Address: ngdumandan@up.edu.ph
Date received: October 22, 2024
Date revised: November 28, 2024
Date accepted: January 3, 2025

ARTICLE

Response surface methodology-based optimization of cellulase production by Trichoderma asperelloides RS1 through solid-state fermentation using coconut coir

Jhoanna Mae A. Minion1, Leo Joseph F. Labitag2, Annie Cita T. Kagaoan2, Adonis A. Yanos1, Sheryl Lozel B. Arreola1, and Nico G. Dumandan*2

1Institute of Chemistry, College of Arts and Sciences, University of
      the Philippines Los Baños, College 4031, Laguna
2National Institute of Molecular Biology and Biotechnology,
      University of the Philippines Los Baños, College 4031, Laguna

KEYWORDS: Trichoderma asperelloides, cellulase, coconut coir, response surface methodology, solid state fermentation

The study optimized cellulase production from Trichoderma asperelloides RS1 using coconut coir as a substrate through solid-state fermentation. A Box-Behnken response surface methodology was employed to optimize three independent factors: fermentation time (3, 6.5, and 10 days), initial media pH (4, 6, and 8), and initial moisture content (80%, 140%, and 200%). Response surface methodology showed significant models for FPase and CMCase activities with p-values <0.0001. Optimal FPase activity (0.762 Uglc/gds) was achieved at pH 6.0, 140% moisture, and 6.5 days. The highest CMCase activity (0.295 Uglc/gds) occurred after 3 days at pH 8.0 and 140% moisture. The regression model showed strong agreement between predicted and actual cellulase activities, with percent errors of 0.74% and 1.83% for FPase and CMCase, respectively.

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