VOLUME 19 NUMBER 2 (July to December 2026)

Wound healing properties of cow ghee and ethanolic pineapple (Ananas comosus merr) leaf extract combination in rabbit (Oryctolagus cuniculus)

Medicinal plants and Ayurvedic ghee are natural products with therapeutic properties relevant to wound healing. This study aimed to evaluate the wound-healing effects of different concentrations of cow ghee combined with ethanolic pineapple leaf extract in rabbits. Thirty New Zealand white rabbits were used for this study and divided into five groups. NSS served as the negative control group; MUP received mupirocin ointment as the standard treatment; and CP1, CP2, and CP3 were treated with [75% CG and 25% EPLE (w/w)], [50% CG and 50% EPLE (w/w)], and [25% CG and 75% EPLE (w/w)], respectively and served as the treatment groups. A single 3-cm full-thickness incisional wound was created on each rabbit's back. Approximately 0.5 g of the assigned formulation was applied daily for 21 days. Gross macroscopic and histological wound healing were graded and compared on days 7, 14, and 21. Results showed that CP1, CP2, and CP3 showed better wound repair than NSS and, in several histological parameters, than MUP. These findings suggest that topical application of the formulation at different concentrations on incisional wounds appears to support wound healing and may improve selected histological indices compared with standard mupirocin.