About the cover image

Photo Title: Neuro-concinnity
Description: A symphony of neurons unfolds, a vibrant tableau of cells reaching out, eager for connection. They communicate through delicate neuronal processes, stretching themselves thin for the chance to send and receive signals. Their conductor remains unseen, felt only in the media that flows around them. It beckons them to shed their solitary, unrealized forms, urging them to seek one another, to harmonize in a shared song. This image captures a network of SH-SY5Y neuroblastoma cells, differentiated by retinoic acid, with their neuronal outgrowths intertwining to facilitate intricate cellular interactions. These slender, fragile projections bear the weight of the signals exchanged among them. Fluorescent dyes illuminate the scene: nuclei glow in blue, somas in green, and neurites blaze in red, weaving a tapestry of life and connection.

Date Captured: August 3, 2024
Location: Disease Molecular Biology and Epigenetics Laboratory (DMBEL), NIMBB, UP Diliman, PH
Imaging System: IN Cell Analyzer 6000 High Content Imaging System (GE Healthcare)
Technical Details:

• Objective: Nikon 20X/0.45, Plan Fluor, ELWD, Corr Collar 0–2.0, CFI/60

• Numerical aperture: 0.45

• Channels/emission wavelengths/colors: DAPI/461 nm/blue (nuclei), FITC/530 nm/green (soma), dsRed/583 nm/red (neurites)
  

• Imaging details: Adaptive software + laser autofocus, 2D image mode
  

• Processing software: IN Cell Analyzer 6000 Ver 7.2 (capturing), ImageJ (stacking and coloring)

Owner/Credit: Kimberly Louise E. Cabral

Suggested citation:
Cabral, Kimberly Louise E. (2024, August 3). Neuro-concinnity [Fluorescence micrograph of differentiated SH-SY5Y neuroblastoma cells stained with DAPI, FITC, and dsRed]. Disease Molecular Biology and Epigenetics Laboratory, National Institute of Molecular Biology and Biotechnology, College of Science, University of the Philippines Diliman. Captured with IN Cell Analyzer 6000 High Content Imaging System (GE Healthcare); processed with IN Cell Analyzer 6000 Ver. 7.2 and ImageJ.